- According to the 10% rule of energy transfer in ecosystems, only about 10% of the energy available at one trophic level is transferred to the next higher trophic level. The rest is lost as heat, used for metabolic processes, or not consumed. - This drastic reduction in available energy at each successive level limits the biomass and population size that can be supported. - After a few trophic levels, there is insufficient energy remaining to support a viable population of organisms at a higher level, thus limiting the food chain length.