- Nitrogen fixation is the process by which atmospheric nitrogen (N₂) is converted into ammonia (NH₃) or related nitrogenous compounds. In agriculture, this is primarily done by bacteria, notably Rhizobia, which live symbiotically in the root nodules of leguminous plants. - Alfalfa (Medicago sativa) is a legume and fixes nitrogen. - Amaranth (Amaranthus spp.) is not a legume and does not fix nitrogen. - Chickpea (Cicer arietinum) is a legume and fixes nitrogen. - Clover (Trifolium spp.) is a legume and fixes nitrogen. - Purslane (Kulfa) (Portulaca oleracea) is not a legume and does not fix nitrogen. - Spinach (Spinacia oleracea) is not a legume and does not fix nitrogen.